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KMID : 0624620110440110758
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BMB Reports
2011 Volume.44 No. 11 p.758 ~ p.763
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Adjuvant effect of liposome-encapsulated natural phosphodiester CpG-DNA
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Kim Dong-Bum
Kwon Sang-Hoon
Ahn Chi-Seok
Lee Young-Hee
Choi Soo-Young
Park Jin-Seu
Kwon Hyeok-Yil
Kwon Hyung-Joo
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Abstract
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Immunostimulatory CpG-DNA targeting TLR9 is one of the most extensively evaluated vaccine adjuvants. Previously, we found that a particular form of natural phosphodiester bond CpG-DNA (PO-ODN) encapsulated in a phosphatidyl-Β-oleoyl- γ-palmitoyl ethanolamine (DOPE) : cholesterol hemisuccinate (CHEMS) (1 : 1 ratio) complex (Lipoplex(O)) is a potent adjuvant. Complexes containing peptide and Lipoplex(O) are extremely useful for B cell epitope screening and antibody production without carriers. Here, we showed that IL-12 production was increased in bone marrow derived dendritic cells in a CpG sequence-dependent manner when PO-ODN was encapsulated in Lipoplex(O), DOTAP or lipofectamine. However, the effects of Lipoplex(O) surpassed those of PO-ODN encapsulated in DOTAP or lipofectamine and also other various forms of liposome-encapsulated CpG-DNA in terms of potency for protein antigen-specific IgG production and Th1- associated IgG2a production. Therefore, Lipoplex(O) may have a unique potent immunoadjuvant activity which can be useful for various applications involving protein antigens as well as peptides.
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KEYWORD
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Adjuvants, CpG-DNA, DCs, Lipoplex(O), PO-ODN
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